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the sanitation and giving me the opportunity to uh open the
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door and uh show what's behind the jane part
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so let's begin by uh once upon a time like that uh something to make c. d.'s name is
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michelle there's a hole in two thousand seven one in from the the the gene part and
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michelle is opinion yeah of uh the infectious disease molecular diagnostic he's the one who launched the first
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uh that is even resistance staff has matured agnostic you should just testing and compromising testing
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and they say that when you create a the during part people lasting like
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mean gene part one two seven seven it's only ten years ago of
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people it's only said that you thought i mean gene one of k. people
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laughing because said that molecular there to speak never the point of k.
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and uh you should notice and everything uh so this is now
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the reality and we have to make an example of
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a disruptive technology that has been your a lot of people at the beginning so we just then quickly some
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remember kodak one for a big budgeted for that big pain
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and go back say never would overtake the feeling like barrett of phone was out the keyboard
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we never worked or not yeah with people just want the good phone not the internet
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but this one is long this one is close to and then this one is gone
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so you cannot read can refuse a breakthrough technology disruptive technology when it's coming
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you have really to analyse the value of the technology is he the best way to include
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that in your day to day activities so this is the same was molecular biology
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so we we ah the next revolution in molecular biology because of the technology we present
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is that okay with you through the system machine and with with jean
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this is our instrument and the for the one who nor
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of a molecular biology in the uh late eighties
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remember that that that time and we want to implement a mercury
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lebanon or split or you have to go with three rooms
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was about hundred square meters and today what you have done was
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their budget would bring that a hundred square meters between eight
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something that that's good or something but yes but it's on the display so this is really a good one
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say you tonight we never know what will happen instance somebody will come use move yeah it more
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with action most uh the mean that users on the the technology but i think we have done the
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vaguely uh a very big step how does it work now or why we do need part
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more today than yesterday and more to more again than today
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let's see what's happened with the patient coming into they must be taught patient is omitted from infection
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but most of the time sample are collected and the tests are sent to the uh the lab
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the lab can be inside your speed thought but more and more the lab is not anymore
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in the hospital doesn't recession the flap in town in countries also so there's a reduction
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in the past or we will only to send a sample from the patient
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the lab it takes when it's inside because people four to six hours
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when there is a subset is action of the lab to take to turn the
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sample from the patient to the lab you take a who's eighteen hours
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so with the patient for example having sepsis you can imagine the
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disaster that can happen because the clock is a big clark
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so the test will be powerful one two three days until you have to find an answer and then you can
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really have a good idea how to initiate the treatment and we consider the else how come they get it
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which point of care so that mean that you move the technology or the testing closer to the patient
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you can see that the clock is much much smaller we're speaking hours and the base
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reception of the that the stick result is about an hour in addition initiation of the treatment again in an
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hour and they have a health outcomes much of much better than what the different between the two
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is for the disease which speak about laughter is the the risk of transmission
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until you do not have isolate the patient buys the patient would continue to this in a uh the disease
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which is a good example was to see them the t. seal and morbidity and mortality will increase
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so this is as wide today i've seen the point of can uh oh what to
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name your patient testing beginning can impact has been demonstrated yeah tones of studies
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to show in the most uh that if you perform the task very quickly and if you can have
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an actionable with that for the patient that is uh uh with the uh very strong added value
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so we all know that the hester cost uh is permanently increasing every window along
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for me recently see after so this is why we have this something business on the flop again
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this is all over the world of canada and the us in france in all countries
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this is what i said the change is is ongoing so we cannot
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stop it unfortunately the something is action is not the right answer
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for infectious disease because we need a quick action that could decision
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uh i was every hour we lose in the sepsis the risk
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of by a best of the patient is significantly increasing
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so the only way to answer that is to the central or something
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i think not or but sometimes think back to the lab
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and that must be done under the supervision of the lab or with the metadata just
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and their need would never stop increase from any reason population just page is increasing so we
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see more and more that the the need to perform some testing in retirement home
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mainly uh one we haven't we know is that shouldn't be p. c. and
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have also has got to be a testing that should be done
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and any ah and we talked about the regular basis you expect that every two hours days of below new one
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so it all as it uh and then then the the next one but that would count just be patient
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and there is uh what would but only what i call our nuclear bomb
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expose monday the problems not even just one when is the multi resistant but that i
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will not under the door and we see that also all over the world
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and the the we must to find the best way to block them twice a also either the patient
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so a part as it is the abbreviation it is for me or we use
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every time there's something with single pass people call that apartment of k.
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so what means papa not part of the people so we can refer to but that which will
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definition this is the one they're using when they go to want to go under and uh sell some tests in the rubbing country
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uh that's a call that i show up for the board
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sensitive specific user friendly rapid rubbers to keep and free
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unfortunately for molecular biology cannot be equipment free because you need
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to provide a tool to on the molecular uh the the amplification it whatever it is i
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will turn work or a p. c. i. and also that about the fun results
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but isn't that one also will need to address saying
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what means in infectious this input after testing
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this isn't actions diagnostic testing that is performed outside the clinical laboratory
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that i mean not under the micro vigil the microbiology um a ladder
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because the control must be there but the person running the test must
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not be inside the microbiology lab can be decent rise another lab
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so this is also an important point this is why we need connectivity between the
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instrument and the lab and then the microbiologist and always ready the the result
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another point that has been said before it's not because in point of care that the
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performance of the test must be low when than any other tested of liquor performance
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must be at least it not to cut to the want octane in the large law oh
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with the big system in the every large level of the uh central as that
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project but what the right answer for the molecular during the sick part of
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care so we combine in our for an instrument record rubber jean
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so this instrument is unit is uh one is flexible you
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can want want to example at the same time
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i can be different sample because today we have only one protocol
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or the test or running on his d. n. a.
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uh it takes seventy minutes seven zero to run the the eight or the one sample
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put it that made it is uh the sample to loading is only one minute order is where is it
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cost effective this is part of the whole the launch so before the pathetic the that's every combat on that after
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connectivity ski so the system is bidirectional connectivity with the major stand
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up to the you including part i want a small footprint
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that's the size of a espresso machine michelle is putting that in the space of the in a so you can uh an idea
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so it's uh it's about the same size uh there's a nothing like and one
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and the if you want to really come printer you can do that but the what's important is a computer is on both
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user friendly with a simple set that it takes about not more than five minutes to train
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a new user with the system because the interface is very intuitive is like my phone
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and for the one one of the us it's what what we call yeah we're compatible
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could be yeah where is the certification in the us that mean that everybody you know most people can perform the test
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so we are we are not seventy five feet away but the discussion with the da told us that uh we could go the if we want
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in front of that we have is a small piece of plastic surgery imagine just uh
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the the size athlete the part the gene park project concept with the c. d.
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so this evening was studied in a different part the part was prototype kuwait recorded the
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part so it's one eight of the size of a c. d. at the beginning
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so in this small piece of uh plastic uh we can deliver the menu and they have
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a actually i loved it and thank y. infection shaken features easy isn't sexually transmitted infection
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this is done he also have the power to detect reason you won a disposable written that that up
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to twelve target so we can work and that that call me nippon they're not allowed to want
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we don't want to go there but the mini panel really morning tradition for example not
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very fun that almost got away panel over budget is on them that uh
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provide the right answer the on set about trying to find the take the and
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then there are the type that we can also the remote onto that result
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and this is important also that a small cap size
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and because a significant people's a lot of time
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we know that in the class about the significant part of the course is going to the waist
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so we are really if you compose competition about one sixth of the weight and the the size so
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this is a significant also reduction in the cost so this is again part of the cost effectiveness
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so the way it works really begin with the sample uh usually to swab
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or receive example with the first and if you see so lucas tool
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that with the shops in the small tool that we provide supper buffet show
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which ones find the pile of volume between a hundred and two hundred like to do that we don't need to be precise
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the system after the and my my coffee they couldn't manage by itself uh the bottle
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then we place on the water and the machine after a running quarterback for the notification place inside
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the machine and one start and after that i went and you need to have the results
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hands on time you can see is really limited is very quick on the two one minute
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so the power of the pie had as it works much the nation but
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that so this is a size and this is divided in three section
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the first one is what you call the road in section where we put our hand with two hundred mike that either the sample
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then we have a universal sample clap here i come back on that and then
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we'll the p. c. amplification with the tree wells one two three which other
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so again we packed our sample transfer to the to transfer to the pile
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and then when we put on and one on the machine just thought immediately machine will spin
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never stops spinning during the seventy minutes so the movement of liquid is
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done by spin and also we use a different level of temperature
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open across some uh some uh choose inside that to uh
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to move the liquid so the the liquid moving aside
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a small chandler hey we call homage invention channel but on this is which
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you spoke about the uh sample prep which is a key aspect
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the sample pack is not only lies in the back of a a purifying the
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d. n. a. the supper prep is also working on the sample itself
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when we were visible journals while we have a lot of makers and
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like this is not the best a friend with p. c. o.
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and when we work with his tool we have pieces of two and still is not
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also the best one was busy at some point you're the most innovative a sample
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so in this region essential amber when have some glass be then shaking which is done by the military
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this well imagine as a sample at the end is no more less whatever that we have
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then by something vacation remove the liquid in this chandler via
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where we have a small too which is close by lifting blue we hate that section not ninety five degrees
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i'm not that if i did we lies the back what about these no one that would resist
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and we also uh open but you and that you will give you the
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sample and put it in the right conditions for the p. c. off
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yeah we're sort of a a small additional aspect which is the process control our process controls as well
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that's the cities so if we can license for buses to t. v.s you can like everything
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so down the peace yeah we re transfer young person to gauge and the body in the
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in the three p. c. awareness and then the machine is equipped reach for the eyes
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so with one by reaching the take up to four o. eight or twelve time
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but for the test i will give my uh more information after we use only
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one where um but we can feed the tree was depending on the type
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so the train example no the first one will be with us would be strap
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it's a test that says perform a different stage usually a for pregnant women
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and she has to go for the testing to be done the thirty five weeks of pregnancy
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and means about five to seven years before that every and uh if the taxes positive at the
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time of the very the room and will receive a shot of an antibiotic of beneath it
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unfortunately i had about thirty percent of the women that that change the status
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was it a will become negative a negative will become positive so the
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value of this task the form of the minus five weeks
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as we know them strong value and this is why we have no another test
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which is called d. s. also directs well that must be it can be and must be perform at the time of the river
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so we have this to test and again this is the same we begin with the sample is either culture
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up to thirty five weeks are the occasional actors well the time of the rate
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that would be shy wasn't you put out here and then we won and machine one of the most of it
00:16:13
so this is the the performance we have today uh with g. b. s. l. b.
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so we have a very good and very uh a very good sensitivity and specificity
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most of the sample we mixed is not that we need the caption miss them
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and every every time we will be checking if the to the sample we would missing
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with another a nucleic uh application technology we always find them positive for the
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problem of the g. d. s. testing you know the p. c. r.
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the value and the quality of the person who will be the plate uh with the d. b. s. because it's not the in either way
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uh with the the yes we have the same will ever have very high sensitivity
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and the specificity was due to this uh the sample that again we found positive with d. n.
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a. the d. n. a. is here so the buddies here but unfortunately it was nice
00:17:06
put it by the capture the the dean so amazing to see
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that uh yeah when michelle there's a whole presented the first
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time to sound on the g. d. s. test the a. d. a. that was the first time the baby a switch
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and said no there is no more testing with microbiology you can go directly with
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the p. c. amplification for the result of that was the first time
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so the performance over very good really run the test uh uh during the last months in germany
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and then the final result in the lab we were close to a ninety ninety eight
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percent of our sensitivity and specificity in real life also because we are there
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said if that's an also said if a is a test
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that must be performed on weeklies to of of tool
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again it's important to generate and provide the result in less than one hour
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because as soon as we know that the patient is positive isolation of
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the patients and then after we can uh perform some additional test
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to a complete the the the decision for the treatment but isolation the for the shoe
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so for that we use on amplify the detection of touch can be
00:18:17
only by p. c. l. so what could have done in europe we want a very large
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study in more than seven country we just use sounds and then five hundred samples
00:18:28
well this is the result so we we have tested our technology against different methods
00:18:34
g. d. f. w. in them that you just said was a billionaire
00:18:37
a a connection g. d. a. g. d. c. t. v. and unix protection the clutches of can we all of them
00:18:43
and the correlation we have with the reference methods when we compare without is ninety nine point two percent
00:18:50
so we can conclude that at this stage we have a very good test uh and clinical performance for our test
00:18:56
when you go country by country so you can see that we have performed when your country should someone
00:19:01
uh has it in specific c. t. are very hard that was compare was billy martin g.
00:19:06
h. unitary it's it's the same arrangement now than uh than france and u. k.
00:19:11
so the overall performance of uh i would have said if tested the it is in the very high
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ranch you can see ninety five percent and close to a hundred percent plus does it does it
00:19:21
that is very important to have a very high sense basically city but you should not
00:19:26
need any positive you can have always of course false positive but the goal is
00:19:31
not to meet any positive and we are really better they another very important point also
00:19:37
due to the technology we use in the sequence you have in the fight
00:19:41
what people and user ace is to have no reason to be and because of
00:19:45
a failure of the system than many do trendy turning that one result
00:19:50
so we have a low uh we're under one two percent even one percent
00:19:55
uh and ways of and that thing that rate which is uh uh probably
00:19:58
one of the best also a a c. available today in the few
00:20:03
well with that system we witnessed thought that was the menu will continue to increase the
00:20:10
next test planning is a new for find a a on a finite is
00:20:15
that would be a step to a panel including except h. the to see and
00:20:20
stop the g. that will put their battery or property between craze that
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the complexity of the test that no one telling after will be this year with the captain resistance until but uh yes
00:20:31
they with five uh resistant gene that would detect it b. c. oaks are in the and you know i mean
00:20:37
and also you can see that the with the panel with the test we
00:20:41
want to develop in the future most of them will be needed panel
00:20:45
and then with the with the part we can do that why because in the correct one we
00:20:50
can put the master needs for the time what one two three in the process control
00:20:54
the second one forty six on the process control and and so the want seven to nine the process control
00:21:00
the pitch once a laptop opinion resistance test would put k. p. c.
00:21:05
in the emphasis controller when one off of savvy meanwhile too
00:21:09
and that's something very important we ha the third wave available
00:21:14
and this is something very important because this is not a stable or status resistance
00:21:21
is always moving changing and we can expect that new gene we can't
00:21:26
and if there is a new one coming we don't need to redesign of a test
00:21:31
we just need to put that in the where available and we keep
00:21:34
the result as they do not have to perform another clinical studies
00:21:39
when i had the opportunity to present that technology to the c. d. c. there were very excited
00:21:44
because this is what they're looking for something open when they can put some eugene and test
00:21:49
what is the situation in the us and not compromising yeah the test because there's no company that
00:21:54
would compromise they're passing by having a new target that can these uh p. two more
00:21:59
so this is the power so of course with that we can do after whatever we want with a with a different band
00:22:07
now we have one machine doesn't mention we have the same protocol for the in it so we can
00:22:13
make different pairs point on the one one so limited investment and some new wanting an instrument
00:22:20
it's an emitted from k. e. o. 'cause it's only in one
00:22:25
and it's a very high i said lower or higher winds of uh
00:22:29
throughput from one sample or two sixty four sample but they
00:22:33
so if we compare was competition if we can one from one to sixty four
00:22:38
and competition depending on the one that will be able to perform
00:22:42
only with one instrument only up to a sixteen test today
00:22:47
so this is for the same price we have a machine that is
00:22:50
capable to do for five time but uh uh competitions capable to
00:22:55
some not why are we going with the point of care enough issues this is so this is a uh
00:23:02
something that the competition that has been published uh in the months ago
00:23:07
by i sent a cessation i'm a concession society for microbiology
00:23:12
and it can be the first time i've here implementation reunite clinic work flow
00:23:17
to incorporate near patient testing uh their patient point of k. testing
00:23:22
come out and then have to everything is going to that but i think this is the major information to do
00:23:28
so in the u. s. now they are changing completely the mine there yeah
00:23:33
moving in that direction to include would be the point of get testy
00:23:37
new routine day to day activities in every or speed than in every lap
00:23:42
is not something to try to to hide it something they want to develop and the we have a big
00:23:47
uh we have a very strong relation also is in the eye and they're very supportive with us
00:23:53
conclusion known subject there is already there upon the most are these with multiple critical difference encore
00:24:00
light variety of a biological sample as mentioned we can lead to rebut
00:24:05
we have the first product available uh in europe but also in the us
00:24:10
they approve many extensions so the next one will be struck panel base e. g. care
00:24:15
about up there we have stat stuff stuff already stuff it p. mechanic say
00:24:20
for maybe and much more our strengths is um you
00:24:25
simple the costs affordable multiplex up to twelve
00:24:29
uh fix immediately want to sixty four foot print very small connectivity
00:24:34
available ways we we can compare with the other one
00:24:38
and also we fulfil debris which you assured a requirement or my computer on the computer and is you can
00:24:45
add or yeah it is you want to chance that an idea on the product need some support
00:24:50
so i just want to say it again they go from our partner who is
00:24:53
with i think the first day and always reactive thank you uh how
00:25:07
ah
00:25:13
oh thank you
00:25:26
if
00:25:30
the infectious diseases can because that much more dentists twelve bacteria
00:25:36
so how do you want to know which bacterial causes detections disease
00:25:43
also and then you're kind of mortgage insulin resistance
00:25:48
i just wonder how you do it like have you seen any intentions to
00:25:52
increase the number of twelve two cats sixty two hundred or so
00:25:56
and it could question even spend the whole day just about the last phone and that's my for them not to do and it
00:26:02
uh this is a big discussion the v. beta one vibe into but they give you a quick a the the way we see that
00:26:08
uh i can tell you that that many years ago i was reading a project
00:26:12
isn't a few metric chip and i was able to detect or but
00:26:17
it was a disaster because we find or both everywhere in or patient so the the way hey we're in point of k.
00:26:24
the bottom from point of canned tests is to provide an answer in one hour actionable
00:26:28
so that's when it might be an easy translation of the what's inside the the the sample
00:26:34
we know that today with um the high a resolution panel available we know
00:26:40
for fun one time that we find multiple target in the sample
00:26:44
so it's so this it's it can be useful for some type of patient in i. c. u. r. e. o. emergency room
00:26:51
but it's surely not where you wanna go with our test is also we want
00:26:55
to lean it they're not just co of uh of uh uh the list
00:27:00
the back because when you see with uh for example twenty four but that on the list
00:27:05
if you go with the top eight uh that's it i would just hope
00:27:08
complete also that the us is also competed changing their mind with that
00:27:13
because uh the the these these very popular this time of test
00:27:18
and the us the the want to use that to eight to nine talk at the same time but not the big one
00:27:25
but that's a decision that we we can do to twenty four used to uh i used to pine free want
00:27:30
but uh do we see a uh an interest not for us also
00:27:35
but for the market probably not is not where we see that
00:27:39
that's that's a big the big thanks

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Conference program

Welcome Note
Gaëtan Cherix, Director - School of Engineering
26 Oct. 2017 · 10:08 a.m.
Welcome Note
Marc E. Pfeifer, Symposium Chair
26 Oct. 2017 · 10:15 a.m.
349 views
Point-of-care Diagnostics: what are the real needs of general practioners?
Nicolas Senn, PMU, UNIL
26 Oct. 2017 · 10:19 a.m.
Recent developments in microtechnologies for point-of-care testing
Philippe Renaud, EPFL
26 Oct. 2017 · 10:47 a.m.
GenePOC, a breakthrough solution in molecular point-of-care testing
Patrice Allibert, GenePOC
26 Oct. 2017 · 11:19 a.m.
Reglementary aspects ruling the reimbursement of laboratory analyses in the context of the compulsory health insurance
Michèle A. Fleury-Siegenthaler, Federal Office of Public Health
26 Oct. 2017 · 2:12 p.m.
114 views
Recent advances in non-invasive diagnostics
Samantha Paoletti, CSEM
26 Oct. 2017 · 3:53 p.m.
How nanofluidics bring diagnostics closer to the patient
Fabien Rebeaud, Abionic
26 Oct. 2017 · 4:17 p.m.
Keynote Session introduction
Marc E. Pfeifer, Symposium Chair
26 Oct. 2017 · 5:07 p.m.
Keynote session: Accessible Bioanalysis for the Developing World and the Point of Care
George M. Whitesides, Harvard University, Cambridge - USA
26 Oct. 2017 · 5:09 p.m.
126 views
Conclusions
Marc E. Pfeifer, Symposium Chair
26 Oct. 2017 · 6:25 p.m.

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