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Influence of Breastfeeding on Immune Trajectory and long-term Immune Health
Valerie Verhasselt
Monday, Sept. 23, 2019 · 3:33 p.m. · 57m 00s
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oh
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thank you thank you for inviting me and thank you for being a steely wage
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so uh yeah why i do i choose essential complicate title
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it's sad because i i think and we will see that ah i don't think we should
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a name and mailing may as a a major on me to develop a just a different entity
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and that's what i'm going to have a now that is a very special period
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of our life but it's not just immune deficient now and developed all the major
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so i think in in trajectory you listed that we come from
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one state to another state and we will see how breastfeeding influence that
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so that's the plan
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ah okay so um a lot of research done on
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on on breastfeeding it is uh uh is on that
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part of life is how the diary it's about the direct effect of maybe some kind of okay okay perfect
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sorry for interpret so that the um
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you can look at the effect of breastfeeding on a real hell's on and uh
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well the life and then it's really the direct effect that you you looking at how breast milk is
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somehow complementing the immune system of uh the nail
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made out in the in the early post metal life
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and you can also look at the long term effect
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and here is this uh uh the notion of trajectory
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how can breastfeeding influence the development of the immune system
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and make you healthy presses unhealthy in many many years later
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so i was that uh by talking about this and finish like that part
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so what do we know about the immune system in early life and i would like maybe
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to ask you a little clays would you consider that a male made it is a small adults
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who would say that now yeah that however i think that uh most of the preventive
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in therapeutic approach of the male make exactly the same as the one for an adult
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and if you look at research in immunology yeah i would say ninety percent
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of the research all probably ninety nine percent of the research is done an adult
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and then we extrapolate all that to the neonatal i think we're doing sinks correctly
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so you all agree this is probably not what should be done in more research on the munich itself
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i don't have that that it's and say okay it's not a real adults it's
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an adult that has no bacteria so you will find also many literature match literature about
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um germ free mice and people just conclude
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that immune says says the immune system maturation
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just can be recap each related to by using germ free
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mice that you call a nice but again it's absolutely not
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true or not i won't go into those detail than many difference that now i really shown
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that if you colour nice and that delta mice you do not um
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get the same as you can and nice and the and i tell mice
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in particular we guiding the i. g. d. but also encased else in many many
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effect assay that at different when you can uh nice an adulterous isn't it it looks like obvious but
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i i guess twenty years of research has been done on adult
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germ free might have and then make the extrapolate so let's not extrapolate
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so let's consider the mailing made as a specific period
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of life with specific a neat and specific a constraint
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and then i think this way we could propose a preventive
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approach and therapeutic i project that really tailored to directorate of like
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so i will uh goals rule um briefly of cause some um specific
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city i think it i really at characteristic of early plus that i live
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so of course that's very obvious the only live is
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still developing and that's why people like to talk to
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about the new nate as a at developing a
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major but do you see there are many other characteristic
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so okay does developing any important um characterise thing of
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being developing is that the barrier and that's fully develop
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i mean particularly the skin is not a a perfectly developed yet and does make
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and the infant very since it sensible to uh infectious disease but also allergic disease
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and also as you all know susceptible to uh address twenties to
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non fiction because the first line of defence is not bad optimal
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uh still developing uh we could consider uh the immune system
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of and they'll make as immunodeficiency free forget we are mammals
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and then in the there is a maturation that in
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terms of production out of systemic in because the antibodies
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something else is still developing in the only life is the micro get that and we come
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from a crises to rile uh environment because
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no bacteria ah describes i some people in the
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you need to row with very rich a microbe rich environments so and we all know
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uh how bacteria i important for the regulation of the immune system in fact i think we can not
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um conceded immune system without the bacteria it's a hole in a system that includes the bacteria
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so they really made and the early life doesn't have that
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established and of course it influence the for the immune system function
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and then let's go to other thing okay this is that grows curve um it's obvious than the
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you know early life we are growing match changing every day while in adult we as somehow in this table
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a phase so all week you compare night though that is stable compared to a
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male made that is everyday changing any particular dot org and i getting a developed
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and development and growth is that's a sit with the type of immunity that is the same immunity
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that is involved in allergic reaction so that beauty for paper for instance from a back norm height
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uh published into sixteen showing that when the l. clearly of the
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line at developing you have i have thirty three that is produced
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and this actor kind is essential to wind use l. c. to active
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h. and and hit the helper to immunity the one involving landlady disease
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so it just let's say by coincidence when you develop being at the same time you have a propensity to have
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th two immune response because this is what you have for repair for development i okay yeah question
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please interrupt me while i'm talking maybe mall to that dynamic
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and the and not their um observation maybe
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that's more speculation but when y'all organ ah on
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the organ of the mail in it i developing you don't wanna have a strong inflammatory response because
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if you you have that kind of immune response you can also have scars and this is certainly but what you don't wanna have
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uh for developing tissue so uh a very strong immune response should be avoided so these
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as some constraint that you specifically finally life
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development that is associated slow inflammatory immune response
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and then come on let's see this poor baby coming on or as i'm discovering such an amount
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of new antigens this never happens in our once we are yeah idols so the immune system of than
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the ornate together with the mother has to cope with tremendous amount of exertion expenditure and of course
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the microbial one but also the full one and
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we we talk a lot about antigen in breast milk
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and then of course inserted for that and you had these uh exposure of the immune system to
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directory antigen which have very important as well for
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a driving the immune trajectory as i like to say
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so and then about the distinctly activity opinionated immune
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system is not an immune deficient is just reacting differently
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um differently because all these new so you
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don't have a memory immune response meaning that
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immune response as slow and that intense so
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the you cannot expect having the same or activity
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no no in that period of life but that just interactivity is also you stated here
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and this is uh i think uh a super important paper coming
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that's what the paper in the same issue of majoring ninety six
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if you inject a uh mice with the various with that those that's you would inject in an
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adult mice to have sick to let it sit thirty take a seat that sector toxic immune response
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so one thousand a a ferris those in than adults mice you have a huge sigh to
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take c. c. i. to toxicity he'd used you do that in the mail made well nothing happened
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so if you only look at this you say okay the immune system of in in it is just immune deficient doesn't work
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well if you'll have then you would say i weaned in increase
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the doe's that's what we do with faxing doesn't work so let's
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increase the those it will not work neither what worked uh is was decreasing that those and you
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can see in this big beautiful paper that you'd a decrease the those by one thousand use that
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having a very potent immune response so what does it mean
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it means that and they'll make is not good at coping
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with high amount advantage and it can be work it can work very well as long as you have very low those
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that's one thing so if you wanna vaccine makes maybe better tried that protocol to very
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low those well currently it's not what is done and i don't still don't understand why
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but you will see that brings me like that
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so um so to to summarise the you know early
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life the immune system in has some constraints some needs
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and since basically cities that we starting to to discover
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and i think this is very important to to have a good preventive interrupted take approach
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so we barrier few immune cells my credit i getting established
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this is all that correct is characteristic of the major let's say
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but also constrain like the gross and this is s. estate wits type two immunity by stuck to what allergy
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lots of the challenges uh the new antigen for microbial ten diet
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and then specific interactivity no memory off you memory and
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respond to low level advantage and and then early life
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is s. s. it'll also with a specific help 'em on was i i like
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to to talk about when they maybe about a gene because it's very interesting paging
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any mean system you meeting is also so different from the adult
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and again we use the same approach uh as uh the adult
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the difference i think is that a paging doesn't have that help and that's a pity so we may inspire
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from the hell that onion it receives maybe one day to provide help also fall eating insist
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so and then eli specific help is breast maybe there was something also is interesting is run
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extensive was that not going to talk about that but i think it's very interesting does that
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think that cover the baby full of gross factor full of anti microbial molecule
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think we main spar from that when we look at breast milk that's today's about breast milk so breast milk
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okay so this is a taught a total artificial graph
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where we forget the mammal nature oh man on the church
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and that there is a need new to rule a period of life where there is as we just heard
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transfer of antibodies and essentially i. g. g. during during the last trimester and comes
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the breast milk that is a provision that i've i ga i was just hurt my yeah
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jury and notice that the that this morning in early this afternoon
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that we'll uh just a cover d. because and maybe also go
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indifference they into the serum and together with these you also get anti microbial factors
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so that's not only that so i think that at twice covered but
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this is so uh this is a scheme um maybe some uh think
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that high put it it maybe it's not only from the get but
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probably uh what's happening is the mother exposed to macro been that get in the land
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we stimulated at b. cell difference asian into pass math aside
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plasma side that will go in the breast milk in secret
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seeming at a minute levine that has specific to microphone in
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the environment and that's probably important also for me to bank
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i think it's good to get to meet from your region and
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not from another region and now the make banquets module is asian you
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would get make from mean yeah well i i don't think the antibodies
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from breast milk in india appropriate for a child growing in switzerland and
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vis versa so we should not forget how breast milk is at
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that it to the environment and how where um dynamic it is
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how are you
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got to okay any question okay
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right okay then i'm what okay sell breast
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milk products and dynamic vibrate my anti microbial activity
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it's not like an antibiotic that will just scale if you back your acts much more than that
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it will add nato allies uh prevent a entry
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of pathogen and of course we had a lot from
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perry about the presence immune cells that are also adapting and you get to huge amount of union sale
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in depressed make when you the mother is infected you have lots of them molecule it
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in don't it's anti microbial activity such as lies as i am i do recently or
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read that it was discovered a bias reebok there a nobel prize
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more than one hundred years ago did not give that name but he found that
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uh back to early to come and it will only already one hundred years ago
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and in addition we have the the capacity to promote the cross of good bacteria so you had a pretty broad
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take the provide to call so i didn't talk about that so you have the anti microbial do pretty batted here
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and yeah so you have uh we will hear a lot about the cross of let's say good bacteria
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you have my corporate that in the breast milk and anti microbial medical so this is a really complex
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a way to approach prevention of infectious disease is not only
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anti microbial it's anti microbial press spree routed plus provide take
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and in addition gross factors so a breast milk is also providing
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helpful um a development of the barrier certainly uh in the room
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oh respiratory uh um the respirator upper respiratory
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um because that but also in that get
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and this is a um an observation make long time
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ago showing that issuing too big a p. p. we'll sales
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we'd set colour strum always more major make you in juice proliferation of those e. p. t. v.
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l. cells and many grows factor in full the batting particular e. g. f. but many out there
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so you have a molecule to repair to stimulate the barrier anti microbial programmatic
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pretty back take i think we can that designed at best treatment to prevent infection
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so this is the allies telling that the very
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first a period of live early live breastfeeding is complimenting
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the immune system of the nail nate and we should
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look at the new innate immune system as these complex mother
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a mother component plus uh they'll makes component and
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then i think it's something complete certainly not immune deficient
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uh yeah it's all are written so that go through but you see how it it's complimentary
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okay so that's it's clear extremely clear breast milk is specifically adapted to the
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needs in requirement of the early life and this is all these uh dynamism
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and the play your turkey and diversity of a of a molecule
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that you find in breast milk that's certainly account for the capacity
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of breastfeeding to be the best way to prevent infectious disease
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and as a game show need this a recent me tendencies
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okay still no question
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okay so now let's go to these other product and
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what's breastfeeding influence the immune chat trajectory and we now
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know that immune system is not only important for infectious
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disease prevention we know that immune system is of course involved
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in disease related to lack of darren's such as analogy disease
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but we know also that you mean system is involved in
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metallic the related disease like obesity diabetes cardiovascular disease so can we
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and i speculate and um and the lies and the study whether breastfeeding we'll
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have a long time inference on these uh trajectories how we could do this happened
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i think the most obvious way and like many people are
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looking at now is about the effect of breastfeeding on my credit
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so if breastfeeding has that many molecule that in three bands at the microbes it will in
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france also the microbial tat and we all know now how mean microbial ties important for health
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so if breastfeeding will shape the microbial to at in early
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life we expect it will influence the long term risks for disease
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what is the ideal microbial it that that's the that's the question and of course
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the ideal microbial to here is certainly not the same as in another continent so
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that's uh we we probably won't be able to jen arrive in relies any finding
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but at least the concept so that's a good certainly a good thing to look at
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and the main research we have been doing is about the presence of anti jenny breast milk
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which is certainly a major driver of immune system um i would say indication
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so yeah you have data that we collect from uh france and australia and
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you have the level of of albion maine and make an to jen in
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breast milk you say it's about if you when we have about fifty percent
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of centre that we collect that way we can get that again to jen
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and they're leveraging those positive samples is around one hundred because graham because graham parameter as
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you probably know detectable appealing in camille is there is in the range of milligram per meal
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so we are clearly not in the same range of uh um uh m. t. gen and not
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and then that's a research we had been doing for uh for now five to five years
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at least we have been looking at whether you find rest director reality mean breast milk undetected i
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we find it in each time you find something in fact it's obvious because you all know that
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i'm most of the thing that you inhale don't go to the land in fact you ingest
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because there is the same because you're a uh um it could tell them and that is
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bringing things back to uh do oral cavity and once a particular is tool to be
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like more than five mike for me that it will and that anyway in the digestive tract
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so what you'll inhale probably ninety five percent that was
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shown in the eighties with the radioactive molecule and that
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in that gets so it's normal that you have in
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fact respiratory allergen here we look at the p. one
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in breast milk and again it's exactly the same uh kind of a range around one
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hundred speaker gram of airmail of uh that p. one you find it in breast milk
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and very similar to in brazil france and australia which have very
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different uh time made a way of living we find the same
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and then more recently we have been looking
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also at microbial anti jen not the microbe itself
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the anti jen and it and we'll tell you later on why we look at that
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and we look at a man are anti jen with stan nice egg
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laying in uganda and we find that you you can have a a
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malaria and t. j. and in the make up of the infected mother
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so you see about a ten percent of my that we'll have some at our at
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and t. gen in the breast milk but you know that you can only get the
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disease because of transmission of the parasites were
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mosquito so this will certainly not infected baby
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but as you will see we want to what has it they want the immune system
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question
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now do you want to ask you question now look yeah but this so to see that's still awake
00:21:45
so i'm okay so let's next what's next next is uh what's happening okay it's good
00:21:54
to look at composition but i think it's important to look at composition and health outcome
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that's so many studies not coming out about composition but then what do you do with
00:22:04
all that i i think it's it's possible it's good to link these speeds 'em outcomes
00:22:11
but then you need lots of money to have those uh studies of course
00:22:14
so then okay let's look at this and let's look at this in my switch
00:22:19
help you to uh eliminate the famous confounding factor or no
00:22:24
if do closer relation that and we can is and then maybe one day translated to the the human
00:22:31
so long time ago now it's a eleven uh years
00:22:34
ago a nice that that match earlier in two or four
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uh i did wonder whether you can enjoy was our turns to breast milk
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and i developed the mouse model in the lab of it
00:22:46
because regulation notes and the that collaboration also would spend ritual yeah
00:22:51
we check if uh uh when you expose elect dating my debt to egg
00:22:55
and to jen with this uh leads to a prevention of as in that
00:23:00
we use again to jen have it as a model for
00:23:03
as in that and our egg was giving a internationally oh
00:23:08
by iris all we check we find it in the milk
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in very low amount has well maximum an anagram for mel
00:23:15
and then we check in the adult hold of those mice if
00:23:19
they was susceptible to allergic response to have that and they were protected
00:23:24
and then we took a lot of time to work translate that to the the human
00:23:30
so here are the results uh that
00:23:33
uh obtained analysing data um if and
00:23:37
i'm doing some measurement in nebraska hold from says and press that in purse
00:23:41
so what we did is that we still can make at three months
00:23:45
and six mounts and measure the level of al demeaning those breast milk
00:23:50
and we look at the outcome egg allergy into children at twenty and
00:23:54
two and a half years so you see this concept of trajectories so
00:23:57
we measured up the contending no fat in breast milk screen six months
00:24:01
and then we look at the channel twenty two and a half year
00:24:04
here at infant that were exposed to press me quits know of that here is press
00:24:09
make a containing of that boss either at three all at six like both time point
00:24:16
you see at at twenty uh a train but nothing significant effect when a hot here
00:24:21
you see that you have now is significant a protection from egg allergy
00:24:26
in children which had been who had been exposed to a true breast meat
00:24:30
of course the number and not very uh and that's efficient you don't see the number here but it's not a
00:24:35
lot we have in total one hundred infant it's not enough
00:24:39
of course it's an observation of study it's not intervention so
00:24:44
yeah that's of um limitation but i think it's the first prove
00:24:48
that um there is hope that you could prevent egg allergy a
00:24:53
true breastfeeding as long as you have egg allergy in the back
00:24:57
unfortunately not all the mother that that eating an egg
00:25:00
have egg in there made a surprisingly a quarter of
00:25:04
monday we never had any detectable detectable egg in the
00:25:07
milk even though that eating a five eggs a week
00:25:11
and uh so that's why we need to know how does
00:25:14
get into there and how to increase the chance to hit
00:25:20
so that was a a showing that egg is good
00:25:23
um but it's not enough in the mice we could uh
00:25:27
um modulator breast decomposition and have made containing tag but no t. g. of data
00:25:34
and this is what you see here this is what i just show you'll uh
00:25:38
egg impress me dresses no egg impressed me and we know
00:25:41
that the may contain t. g. of daytime the mice also
00:25:45
now if we know tries to jeff that time there was no t. j. bit anymore in depressed make of the mother
00:25:51
that's in the mother that's important you see that even though
00:25:54
you have egg antigen in to make you don't have any protection
00:25:58
so you you say that again that compliment charity not only for the very beginning of
00:26:03
light but also for that the indication in the long term outcome you need to have
00:26:09
was the anti jen but some cough factor that will that then they'll make is missing
00:26:14
and then the mode is providing that on not and will help
00:26:18
in dictating it as immune turns so you you see here for instance
00:26:24
already different a long term outcome just by modifying a few factors you
00:26:30
have no egg you have no t. g. have made that you see that the
00:26:33
long term outcome is absolutely not the same just by manipulating egg and tedious beta
00:26:38
so we can understand why there was so much difference and in terms of protection from allergy
00:26:45
and then uh what we also find it was that protection less a seat which t. hate to help
00:26:51
the one uh sell develop and when you mention how important is development of t. l. to win immunity
00:26:58
uh we think that a macro pure ties involved in that and i'm not
00:27:02
a maturation to what's teach one set in the other factor in breast milk
00:27:07
contribute to the development of to help the one okay
00:27:10
so egg is good it's not sufficient unit cost factor
00:27:14
in t. g. advertise one of those cool factor that we showing demise the to be proven in the human
00:27:20
and then uh we did a lot of research
00:27:22
of a antibodies um impractical it i. d. a. n.
00:27:26
i. g. g. and we wonder if and my there has been exposed to the egg which is the normal
00:27:32
situation that into reality in the real light that very few mother that and naive to x. so that all
00:27:37
have antibodies i. g. a. n. i. g. g. two of that that's a change something yeah it changes that
00:27:45
so what does it change its shape that if you have a i'm
00:27:48
not in the breast milk the anti g. n. n. i. g. g.
00:27:53
then you had the formation of immune complex and these
00:27:56
immune complex at transfer to then at l. f. c. receptor
00:28:01
they will be captured back antigen presenting cells and this
00:28:04
time the team just teach oneself but fox peachtree t. rex
00:28:08
and now the protection will be extremely strong very long a long life
00:28:13
and we have a protection that that's deceased each one but with folks peachtree to
00:28:17
wreck and come and um the initial co um i was suppose also to come here
00:28:23
and she also recently has had the same observation and as we did
00:28:28
plus additional experiment shrink the role of f. c. around on antigen presenting cell
00:28:34
so i guess you will ask me about the i. g. a. we check the role of
00:28:38
i. g. a. and in the mice be inhibited the presence of i. g. in the make
00:28:44
by giving an antibody that prevent the migration of b. cell
00:28:48
to the memory gland and then we check we had no
00:28:51
like g. eight of that and we still have to protection
00:28:54
so at least in the mice i. g. g. i. important
00:28:58
and uh uh in the in the human them um i have preliminary de cat
00:29:03
but i can't actually good that today next i sell t. g. f. i. g. g.
00:29:10
and in fact i mean a so if you'll expose a mother
00:29:14
tool of factoring the whole lactation eating use protection but if you
00:29:18
expose them out there to the egg anti jen only the first
00:29:21
week of life you do not in use protection and we wonder why
00:29:26
and the y. yeah um was the relate to to to get barrier
00:29:31
we compare the um get very yeah in one one week
00:29:34
old demise was history week old what a three week old nice
00:29:38
the mice a bit premature compared to the human and so
00:29:41
they get is really not fully developed and so you see that
00:29:45
that's three week old all it is the one week old macs
00:29:49
that has been exposed to press me containing supplement of that community
00:29:54
so you can accelerate the maturation of the get italian
00:29:58
and make it more um tight and more developed with supplement of
00:30:02
i terminated elect eating like that so you in france that that barrier
00:30:06
and you can also influence the capacity to activity cells to what's
00:30:11
a regulation induction by giving that tammy may so here you have
00:30:16
t. cell activation that you measure in the five days all the nice receiving again to jen
00:30:22
to the milk and you see that when that five they all they do not respond very well
00:30:27
unless they had receipt supplement of act i mean it would a breast milk
00:30:31
no t. cells in the one week all the mice can already respond
00:30:35
and be active eight and mount tolerance and this result um in that
00:30:41
all i don't mice you checked now they reacted eighty two aggie you challenge then we egg
00:30:46
the one that way exposed during the first week of like than
00:30:49
not protect from allergy but if they wake spit exposed to egg
00:30:53
to get the weeds that have meaning the first week of life then they get protected
00:30:59
is that a little so okay
00:31:01
it's more complex just show like that in daily life inducing a row turns doesn't work very well
00:31:07
probably micro get that play a role what we have highlighted is the role of fact i mean a and then in it it's
00:31:14
physiological e. vitamin a deficiency was very low level
00:31:17
in early life in fact anyway you can accelerate majoring
00:31:21
in early life and gave fact i mean a supplement to their mother and then you increase the barrier
00:31:27
of the mailing a and you increase the capacity of the the only
00:31:30
to handle anti jen and pro and use a regular to reunion response
00:31:37
so from this experiment you see that's what's happening and if briefly skim it always came back scheme
00:31:43
schematic way of what we found in the mice most of the experiment if you in the human
00:31:49
so if you all exposing eliminate two of albion maine
00:31:54
verses null of l. b. mean you will not change match
00:31:57
but now we show text expose the they'll make two of that be mean and milk a factor in like
00:32:03
tape we have been looking at it teach you have data you decrease the risk of allergic disease later on
00:32:09
if you expose to of that and i ten mean it was
00:32:13
that only during the very first day nothing happen unless you add
00:32:16
fact i mean they you can use turns vary from the very beginning of life
00:32:21
and then if you have i. g. g. specific to of l. b. need to get rid of
00:32:25
that be mean you have immune complex and you can also use in utterance that is very profound
00:32:31
so you see here just by changing the amount of if you factor
00:32:35
you can totally change the outcome and the susceptibility to a lady disease
00:32:40
still if um i would answer to a question is the mother eating
00:32:45
and mega will it prevent allergy disease well the lines where is it depends
00:32:51
and you see why this is the the answer that people don't like they would like a black and white but no that's how we did
00:32:59
and the um it took to uh finish uh i
00:33:04
will talk to you about the the no t. uh
00:33:08
a legend that is uh that p. one that is that a legend from has this
00:33:12
night you remember we find it in the breast milk in similar amount as advantage and so
00:33:18
um we would expect and we thought it would
00:33:22
interest germans having dopey one a legend from has
00:33:25
just like in the made and it would interest darren's but no our experiment in the nice show that
00:33:33
if an mice is breastfed by your mother without having any has this
00:33:37
nineteen them make verses having how's this might knowledge in in the mic
00:33:41
well then you increase the allergy risk when the mice is adult
00:33:46
so you look at the mice that has six eight weeks old and
00:33:48
you find out more susceptible to uh allergic response to house this night
00:33:54
that is in the nice and that's also true in the human weights not call that it's an association we find again
00:34:01
when you compare the respiratory knowledge you risk in five years all
00:34:04
children during that the first five years children that had been expose
00:34:10
to a basic containing how's this might originate presses exposed to make that do not contain
00:34:16
in the house this might not age and then you see that
00:34:19
you really increase the risk once they were had been exposed to
00:34:23
house this night allergen so yeah oral exposure to an anti jen
00:34:28
and doesn't mean you into stern's even if breast milk is there
00:34:33
and probably that's good uh for infectious disease prevention so now
00:34:37
indeed when you see that this is certainly not not that good
00:34:42
and yeah before moving and yeah this is not good and we need to understand why we need to know what we can do
00:34:48
and that's what we're doing at the moment in in mice so we are trying to find what is happening
00:34:55
how is the immune system of the mailing eight activated by speaker graham
00:34:59
of the house this night and as a yeah he was mentioning you
00:35:03
have intact pertaining breast milk and surprisingly you have proteges from has this
00:35:08
might that can be fined in the breast milk so imagine the whole tactic
00:35:13
project of all that i that's extremely hard to convince the reviewer that this is happening
00:35:18
um that's it is happening so two years of review
00:35:23
um maybe it will get a review of published when they
00:35:26
so i'm very happy to share uh so the press days
00:35:30
and in the breast milk i can tell you we had one hundred experiment to prove that and this
00:35:35
is it is serving that get very it's increasing that
00:35:39
the permeability is active rating that it p. tell yourself
00:35:43
liberating site the kind that in important for type two immunity
00:35:47
development and favour ring t. helper to immunity and not a protection
00:35:52
so uh can that also tell you that having has this nineteen breast milk we can also favour from dot e. g.
00:36:00
uh_huh hopefully you will see that published so that's uh and about
00:36:05
has this night that it's certainly not protecting and we are working
00:36:09
on how to decrease the level how just might impress me how
00:36:12
to nurture like they put is activity to to go in that direction
00:36:18
but then yeah let's go to this yeah when you have a tiny amount
00:36:23
of a ninety jen that stimulate the immune response well this is sexy nation
00:36:28
so can we extrapolate from that and say okay that's not good
00:36:32
for allergy but not imagine you have microbial anti jen in breast milk
00:36:37
and a pair of question along time ago when you vaccinate a mother
00:36:41
a woods rubella vaccine you fine rubella uh you you you find it
00:36:47
immune response in the child also to rebel us thinking that something is happening and what we
00:36:53
think maybe happening is that the anti genotype transfer in breast milk even need the chinese that infected
00:37:00
only the anti jen could be in the best way to activated immune system be all the
00:37:06
contact that that are required to prime the immune system so this is a study we yeah
00:37:12
we want to do uh with a term that's it when in again
00:37:15
that uh with him we have been looking at breast milk of that infected
00:37:19
mother we know about ten percent of them are the head man i
00:37:22
rented jen ninety percent do not have any would like to know the outcome
00:37:26
at the children that are exposed to men are anti jen beep will they be protected um no that's uh
00:37:33
we need funding so we'd like to do that so um
00:37:38
we can conclude and i i think you'll agree we we can
00:37:41
conclude that the in breastfeeding has is clear impact an immune
00:37:46
system function in early life in it's critical to prevent infectious disease
00:37:52
and it will also uh inference that immune trajectory
00:37:56
certainly in case of allergy disease we have now accumulating date that that
00:38:00
according to the presence of a legend in ca factor you can have a
00:38:05
more or less energy disease susceptibility and of course all
00:38:09
the research we i'm doing yeah with my team is two
00:38:13
now what can we do to manipulate does breast may come
00:38:16
position to decrease the risk in the long term fall knowledge
00:38:21
so this is the team these at this open nice people that uh
00:38:25
join me in a western australian press the most isolated sitting do well
00:38:30
um but you see i'm not isolate time with the m. i. q. that i. q. has been working
00:38:36
with me for ten years in france and uh did did the chant i'm moving to well we'll try yeah
00:38:42
batteries yeah the the the show a few years mean from us and for her p. h. d. and then uh
00:38:47
i sixty to get her and her family also coming uh uh with me
00:38:52
and she she had moved back to brazil but then join the team and
00:38:55
i made the ca in japan she was uh in new zealand finishing oppose
00:39:00
that and then she also join the team and the we yeah male friendly
00:39:05
so uh a jacket or and uh recently uh join the team
00:39:10
and we're very happy hating him a working on a also how um
00:39:15
how to any beat has this like put is activity in here the collaborators in particular says and press cut
00:39:22
who gave me access to press caught which is a huge amount of
00:39:26
where the which i skip thanks to put people that a mounting press caught
00:39:30
and here's to mess a going a super motive eighty nineties that's research and again that
00:39:35
uh calling me a probably every day to know a way i um what am i doing it
00:39:41
and i'm very happy we will have a student from him coming up
00:39:44
uh to uh to purchase and i also thanks full to allison has
00:39:49
increased foundation emmett forgot to mention at the beginning that funded descent famous
00:39:53
chair i'm very uh fortunate to have and i thank you for your attention
00:40:03
ah
00:40:04
pretty rude would you call or to remove someone from from russian military short plutonium
00:40:12
i don't remember lose certain return are questionable you'll
00:40:22
so which one oh
00:40:26
how are you well
00:40:37
uh_huh so
00:40:39
oh well all of us or
00:40:49
it's flat or
00:40:51
so awful
00:40:55
yeah
00:41:01
yeah i mean is that seemed using uh actually uh it's
00:41:05
interesting protection um so and when you uh it's come it's
00:41:10
yeah so i can come in later on for house that night but fall outside the main
00:41:14
uh we've did western blot analysis and we find intact of that remain another have and that
00:41:20
and we did the and then lies if it was bound to i.
00:41:22
d. g. and we find that uh when the mother at amy nice
00:41:26
here in the mice uh the most of the of alvin is down
00:41:30
to a. g. g. now in the human that uh we have um
00:41:34
we found of albion maine and i. g. g. n. i. g.
00:41:38
a. and we try to measure immune complex i uh after uh
00:41:44
few years i would say i have stopped doing that because there
00:41:47
was lots of course we're activity when we do immune complex analysis
00:41:51
and the western but was not sensitive enough to detect of al remain in the breast milk
00:41:56
so that's one thing now we guiding that has this might of course if has this might allergen
00:42:02
i've onto i. g. g. o. twenty eight may change in the mice model that that i'd show you
00:42:07
uh we find that i'm the user model by the mother i expose there is
00:42:12
i. g. g. do reside ga and however there is that increase the risk of energy
00:42:17
so that's uh the cost of all over or
00:42:25
or remove file uh_huh oh
00:42:35
yeah yeah we did that
00:42:39
sure yeah we did that we did tell filtration we measured are
00:42:46
how i damien complex it's bikini and most of the immune complex at big union complex
00:42:52
we try to to see the mail nate with losing income place
00:42:55
to see but that that's some immune complex that that protective or not
00:42:59
but it's absolutely not the same uh when you do that um
00:43:04
yeah you don't really have protection other things i'm missing it and it's that continues breastfeeding we when you sure
00:43:12
for for ah yeah 'cause you know i can answer that uh the amount have very low so it's
00:43:21
that yeah if you talk about human about mice in the mice i cannot
00:43:27
get enough milk and in the human that um i i haven't done it yet
00:43:36
yeah oh whatever you choose oh or protein when you show
00:43:43
uh_huh aren't through yeah okay yeah why do you use are
00:43:47
probably due to induce you you're towers rubber house no protein
00:43:52
analogy which is far hormone in the first year of law or even worse for it
00:43:59
okay uh so uh uh they're few uh and so it's that
00:44:03
so first i i in australia of for instance in the prescott i showed you that
00:44:08
the main energy is egg allergy so i think i got a. g. is quite common
00:44:13
tool we have done also analysis with its cold milk
00:44:16
um with a detectable abilene and then we find that um
00:44:21
it's not that protecting as good as egg is is i don't know if it's that one you your question
00:44:27
so why we use of that even that's because i'm in the middle ages then you have
00:44:31
many tools to look at a response to a value means so when you want to mow
00:44:36
model lies the question it's easy to use of aluminium you have a lots of tools
00:44:41
so that's why i use of albumin as a model for how fall wrist director allergy initially
00:44:46
then we moved to the human and i think i got a. g. uh you may have to notice in paris
00:44:52
caught that fourteen percent of children that analogy kept when you're in a high risk or hot
00:44:57
and among them and they went nearly all egg allergic so that's it is really very
00:45:02
common it's true now of course week we need to extend observation to cull milk and that
00:45:08
that's on on the way and looking at correlation between
00:45:11
that and the collective in and co milk allergy route
00:45:16
however will so do presentations always of you are with descriptions apart questions from
00:45:25
the work and works too well printed
00:45:28
useful tool to use mm mm change something
00:45:34
mm himself to do some room talking about unix how many people today
00:45:41
what do you you know how many years would be too much
00:45:46
yeah yeah they they beep timer is a seizing parcel soul and
00:45:51
she's really uh trying to answer your question that that they already answers
00:45:57
so the cheap at least the quest idea where um but it
00:46:01
was an intervention trial my there were receiving zero back a week
00:46:05
for us is one or two or three or four or more than five in australia bill off accessing the eat everything
00:46:11
they to x. so i they eat a lot of things so damn there and alive then into different group the presence
00:46:18
so they tend to be a doze a relation them all a demo chance you have to detect the however
00:46:24
the when eating fat the group eating five days a week there was still mother that
00:46:29
had no egg and it's a high proportion it's about one third and and yeah that's it
00:46:34
the one that avoid like so it was a randomised control trial they had strict instruction this is like this
00:46:40
from a uh when you have zero bag to side of the mother had a detected in the breast milk
00:46:47
so when we talk about avoidance uh you don't know uh oh where does it come from but there
00:46:53
now about pregnancy again to be timer is doing a a clinical trial called pregnant
00:46:59
she's exposing on not my that to egg and
00:47:02
met during pregnancy and lactation and we see the outcome
00:47:06
but it will take a few years i'm going to go for criminal fibres no yeah
00:47:12
often reforms wonderful or or or is remembered
00:47:16
you highlighted summit's problems animals worthwhile to me
00:47:20
yeah i'm oh sure tolerant which is problem
00:47:25
should be immune responses from one to concentration
00:47:29
who wants to work probably i mean you have to remember or there's wonderful
00:47:35
our offices that perhaps what supplementary yeah you will lose or cruise or uh_huh actions
00:47:43
i'm sorry point one dollars no or corrupted over remarks probably
00:47:48
robert prevention or whatever your remote controls holders teachers
00:47:53
mm sums of course also all you remember scream and yell to mute or consumers
00:48:03
our problem you want to comment on this or come yeah absolution situ news or forms one or
00:48:09
yeah exactly thank you so that that's not a question lies about tight anyway
00:48:15
a surprisingly we find that if in fact i mean a to like taking my there was not in use
00:48:20
estimating folks petri t. rex that c. h. one sense so that's one
00:48:24
thing is inter from ghana dependent protection um that um uh in the
00:48:29
human i've found tool uh studies so there is one that look at
00:48:33
the nasa station between level of a fact i mean e. in early life
00:48:38
in the in children and that as my race and they find an association that goes in the same direction that
00:48:45
we see the one that had a very low fat i mean a level in a life they worked more risk
00:48:50
that's an association that's there was an intervention trial in given the cell i think
00:48:55
um where they gave the fact i mean a but that's simply maybe sour two children and they found that
00:49:02
when given fact tammy may in the first day of
00:49:05
life you had an increased risk of allergy so from either
00:49:10
explanation because your is finding explanation you like that's
00:49:14
the explanation i like is that in the maybe sell
00:49:18
uh interesting fiction now and image so it then you're giving flight i mean a an inflamed because that
00:49:23
in fact i mean i think is just as the literature is suggesting promoting is it excellent enjoyment
00:49:29
so if you have ongoing th went to help uh seventeen it in fact i mean
00:49:32
a you will increase that so i i think it there was still a some um
00:49:38
i think it's it's possible that fact i mean it could help but no one is this thing that i
00:49:49
i i
00:49:53
uh_huh uh
00:50:02
your comment about our all the reviews as personally or
00:50:06
someone questionnaires was the submenu points you couldn't reviewer course
00:50:10
but these were also just the views were premature
00:50:15
and the reason i ask a question is what we're calling sweep your to promote
00:50:18
all just don't know what your story or she has seen was the topic true titles
00:50:25
control over what you what kinds of cure you compared to what we were probably just
00:50:33
okay so i'll ah you you like the the date down a city street because it's really
00:50:39
to developmental because we find that you think that houses michael's okay you're supposed to be worried about
00:50:47
one product yeah one double yeah exactly an agreement reviews but yep um well i was actually
00:50:53
yeah yeah also question the girls whose whose is
00:50:57
it possible for two years ago probably really yeah
00:51:01
or am i thought well recently in the news person will probably use isn't
00:51:07
that something good pollsters yeah yeah you produce with one will still be working
00:51:14
or some reviews responses is that this particular observation scene
00:51:21
yeah i i'm i'm wondering exactly the same question i would like to know how is that it's it's it's really
00:51:28
producing interested we don't usually yeah it is indeed and and now i realise i did not answer to the second part
00:51:34
of question regarding upper mentality i don't remember the name of the french press the
00:51:41
course yeah you actually sensing at the outset
00:51:43
that permeability grimly and flattering yeah so uh
00:51:48
yeah everyone thinks that since it is a should occur through the skin
00:51:51
i think since it is a shame can certainly appear to to get
00:51:55
um and and and certainly in early life when did that
00:51:58
barry is that uh the plot and when you have insults like
00:52:02
how this might all the molecule that after the the barrier on
00:52:06
multiple come recalls huh marshall for references or uh_huh should work on
00:52:12
part of our remote so obscure yeah only one factor question jurist
00:52:23
uh_huh should do you do to to use more or or it got dark room room for one or two or three version
00:52:31
when the contributor rheumatoid reserve for her
00:52:38
yeah i'm sure they must be data on that i i'm not sure where
00:52:43
that uh they are that's over there is lots of research no i nicer to train the context
00:52:47
of i. b. d. for instance and show people have look at like forget that but i don't know
00:52:54
i'd like to ask how we were better niceties off relate
00:52:59
to the i mean the fall into rule shrink shall we
00:53:04
do you recall my amp to all the room with a little too close or else bothers me
00:53:11
all all are are mainly of goals overcome all the only yeah oh no
00:53:17
well yeah if you look at the amount of anti jen
00:53:22
hello other jen you're having breast milk it's a very low
00:53:25
and if you look at those data that have been generated in in in the mice
00:53:30
certainly is that the male mates is really more happy to respond to low amount and to mount the
00:53:37
correct response with low amount so to me press make is ideal to prevent allergic disease because you have
00:53:44
all a diverse uh amount of product it's you have everything all what the model is eating is then
00:53:50
it is they are not the depending of the model but you have much chance to have a panel of allergen
00:53:56
while now we are proposing to those baby that that three four months to eat nuts
00:54:01
to eat kiwi to eat so many thing i don't have the feeling this is really
00:54:05
uh at that it's to that period of life however breastfeeding is not putting cutting from allergy
00:54:12
so you have those are a gen but it's not enough
00:54:15
so maybe depress me composition has changed it to choose to protect
00:54:20
but the maternal diet has changed doesn't have maybe enough tribe or maybe doesn't have a the goodbye corporate and we lack
00:54:26
the press make now is lacking the cost factor that's possible so let's
00:54:30
try to keep all those allergens in low amount plus what is needed
00:54:35
and let's find the what is required to get the good may come position in the room or simple derision i would
00:54:42
love to or should not really should be bloom semantics of
00:54:46
tools to read what was it didn't lead responses no no more
00:54:52
yeah but i'm not sure right of whatever it was
00:54:58
a paper also showing that would that be features now
00:55:01
you have memories and then yes i would like to order for i say oh no or little memory thing
00:55:08
compare yet to protect yeah uh_huh circuit question second
00:55:14
she was user or the dinners biased towards old age
00:55:19
so i stay protected in order to call in the context of
00:55:25
roy determinant drop or or dropped because the bars dreaming
00:55:31
but prior exported engine or blah or or or so i'm not sure
00:55:39
from our partners or bars or storage
00:55:44
yeah i i think that bias to no tolerance that bias it it has also served under ours
00:55:50
no it's hard to have our work problems and that biased i think to allergy because of the grows
00:55:56
and the grows into repay make an isn't uh i sit with protection of a molecule that's important
00:56:02
file a g. i. like this isolated street and we know more and more that type to immunity
00:56:08
is an immunity that is involving grows so it's by default something that is wrong
00:56:16
oh sure work or or we should read across one where all callers
00:56:23
we work toward women and warren fractions of a few whoa
00:56:28
opted for him and the contractor so
00:56:35
different factions rooms for cruise over it
00:56:41
a virtual call factors would be more important problem you are one
00:56:46
bars for the process or for sure that's why everything that's awful
Conference Program
NNIW94 Opening
Dr. Natalia Wagemans, MD
Sept. 23, 2019 · 9 a.m.
Dedication to Lars A Hanson
Pearay L. Ogra
Sept. 23, 2019 · 9:10 a.m.
136 views
Key Note Lecture: The Evolution of Lactation in Mammalian Species
Olav T. Oftedal
Sept. 23, 2019 · 9:29 a.m.
678 views
Immunology of Milk and Lactation: Historical overview
Pearay L. Ogra
Sept. 23, 2019 · 11:01 a.m.
450 views
Common Mucosal Immune System
Jiri Mestecky
Sept. 23, 2019 · 1:03 p.m.
444 views
Immunomodulatory components of Human Colostrum and Milk
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Sept. 23, 2019 · 1:57 p.m.
481 views
Influence of Breastfeeding on Immune Trajectory and long-term Immune Health
Valerie Verhasselt
Sept. 23, 2019 · 3:33 p.m.
426 views
Milk and immune competence in prevention of allergy and infections
Carine Blanchard
Sept. 23, 2019 · 4:31 p.m.
511 views