Player is loading...
Role of microparticles in portal hypertension
Prof. Dr. med. Pierre-Emmanuel Rautou, Hospital Beaujon, Departement of Hepatology, University of Paris Diderot, France
Thursday, Dec. 6, 2018 · 6:04 p.m. · 48m 03s
Embed
Transcriptions
Note: this content has been automatically generated.
00:00:00
q. roman chinese or for this very kind introduction this is uh the for me um great
00:00:05
great pleasure to be here a wheeze you in and also a great tone or
00:00:11
uh in the steaming including a highly under
00:00:15
a well known a better d.'s
00:00:18
and and also this is for first time for me here though so i'm happy to
00:00:22
see where you guys are working so uh thank you very much for this edition
00:00:28
uh i will share with you uh i don't know if it's i can use this one or
00:00:35
no okay i would share with you uh uh okay so i'm in a
00:00:40
data on a micro physical saying uh in general or extras to leviticus
00:00:46
so on my talk will be divided into three parts i will first uh give you some background
00:00:53
uh about extra citadel visitors and i sing this is worse uh having
00:00:58
some ideas about what is what are these extras love because because
00:01:03
they are rising stars these expressive dramatically up here
00:01:08
uh as promising by your markers and we'll talk of that in the
00:01:12
field of liver disorders and uh also play differently a crucial role
00:01:19
in many does alderson we see that in the field of a series is
00:01:24
so let's start with some background about these extras to law physicals
00:01:31
expressed in america's include exerts alms very small extra syllables acres
00:01:37
this is on the left side they have a diameter ranging
00:01:41
from forty to one hundred and then no matter
00:01:44
a bit bigger microbe physicals having a diameter of one one two one micro matter
00:01:51
and then you can be your updated body is having a diameter of one for my commenters
00:01:58
to give you a an idea of what are these what is
00:02:03
the size exerts some is about this time of a virus
00:02:08
michael basically this is around the size of bacteria and
00:02:11
uh uh putting buddies have a size of projects
00:02:16
oh these actresses rather seekers uh ah are different by is the size but
00:02:21
also by the way z. are released if you take a exams
00:02:27
they are released from uh maybe we could all buddies here you can see on the some
00:02:32
basic on that topic and up into a within the cell into metaphysical of bodies
00:02:38
and this is ridiculous bodies fuse with the past my membrane releasing the
00:02:43
extras in last place this is very very small basic ups
00:02:48
then microbes acres micro physicals are released by cells
00:02:53
in the extra salami jen following typically
00:02:55
to stimuli user up to these early steps off up those ace or activation
00:03:03
a good example of cell activation which is uh quite we're name is abruptly activation
00:03:10
you likely know that black cat wins are resting have a round shape
00:03:14
they are circulating in the broad but as soon as you had an injury to the vessel sent to completely change
00:03:21
the shape and they had so the parts that are able to uh a block the injury and this
00:03:29
uh extremities easter but can we need extra syllables thickens
00:03:34
so stimuli for a micro physical really is is
00:03:38
a activation circulation or uh up to these
00:03:41
so the sizes open one one michael matter and they expose that there's a phase in this is important for
00:03:47
the function expose i i cannot make forcefully beats uh
00:03:52
which are a negatively charged a forcefully bits
00:03:57
even bigger this is a a pathetic but these are really is of course
00:04:01
my sadness and uh going up those these but they stages of
00:04:06
operators is the cell is dying and the new clues is from abated
00:04:10
and you can see the n. a. wheezing z.'s updating buddies
00:04:15
this one are a bit difficult to study because if you take friends on the block
00:04:20
we've got a sizing data packets so it's difficult to discriminate the same from a pet sets and therefore
00:04:26
you will rarely see papers on this about that big bodies i think mainly for technical reasons
00:04:32
and also for question off along because are quite rare in the broad for instance
00:04:38
so the rest of my talk will be mainly on uh this actress allow visitors
00:04:43
exerts um so and a microwave signals and you will see that the separation
00:04:48
in size is quite arbitrary and there is an overlap them but to see that in two seconds
00:04:55
so these are just a lot of the goods we can detect them so many techniques and i'm showing you few techniques
00:05:01
for you to understand the negation of fruit which one and
00:05:05
also um the positive aspect of each one flow centimetre
00:05:11
percentage of this is just like for said you know this is a you've got
00:05:15
a really clean us the right and uh this week we go through laser and then
00:05:20
depending on the size uh you've got a different reflection of the uh laser
00:05:26
and you can it i mean from that the size of the event the physical that goes to the laser
00:05:32
and we've got calibrated needs and we can we can mean a gate of events
00:05:37
uh having a size of micro physicals namely open one to one michael matter
00:05:43
but the problem with rosetta mitre is that we can
00:05:47
barely see smaller micro physicals don't one between
00:05:51
open one two three my computer with new generation it's easier but this is an indication
00:05:57
but the advantage of frustrating batteries that you can use on tape up
00:06:01
a body to let their each micro physical and then did
00:06:06
i mean the sell off arranging of micro basic years because i
00:06:10
told you that these microphones gives out the right from sense
00:06:13
and the force ah my critical derive from about aside from white blood cell from a richer
00:06:18
size except ah so with prostate imagery we can that i mean the sort of origin
00:06:25
there are also techniques uh for instance uh then a nice uh like scattering
00:06:30
which is based on the the brian movement of a very good
00:06:35
a a physical memo makes a little bit and there is a relationship between the size in the uh movement
00:06:42
and with that again you can that i mean with the high accuracy the size and you can go to very
00:06:48
very small size you can see this inanimate or we can go to a even less than ten an animator
00:06:55
this is a preparation of a extra syllables acres and you can see the quantity
00:07:01
here is one michael matter open one my computer and therefore here are my credit cards
00:07:08
but you can see that uh here this is one
00:07:12
three two one my computer namely this population
00:07:16
and you can see that it represents only only very small pop that prozac dimitri can see
00:07:24
um you can also see from that that the separation doubt two peaks um
00:07:29
to be imitation is on something like a fifty nine an utterance of all these
00:07:34
different between it was um and uh michael basic you'll is a bit arbitrary
00:07:38
and you should you in fact we are using a soup of extra sullivan suckers
00:07:43
and uh rick review over do not like so much now that we use microwaves get all examine prefer that
00:07:49
we use the damn extra syllables acres even sometime large or small but be cautious wheeze is differences
00:07:59
so did the advantage of that is that we cannot uh no the set of reaching off excess another ticket
00:08:05
we can use that technique we developed the technique very simple
00:08:08
which is based uh on the filtration off the plasma
00:08:13
and take a plus model we find try distressed mass who pours
00:08:17
we remove a microwave because then we can do one eliza
00:08:21
and fight rate again through very very small balls and then remove uh exerts arms and
00:08:27
from that we can deduce uh the the uh marcus present on visit cuts
00:08:33
so i'm i'm done with this technique technical background but the purpose was to show you
00:08:39
that there are many techniques each one has is its advantages and it's this advantages
00:08:47
where can we find extra syllables acres of course from
00:08:51
from the broad it's known from a four years
00:08:56
but also it can be detected in also by the
00:08:59
free it's in the study via even individuals free
00:09:04
in a year in in signal creating joins also
00:09:08
and you might know that remote reduced our uh injecting the p. r. p. you
00:09:12
know it's our uh in fact i think p. l. p. this is my
00:09:15
critical ceases project reach plus my that contains a lot of activity packets and i
00:09:20
think the room they in fact inject a lot of a micro physicals
00:09:25
but also in most source and you can weakness i in a
00:09:29
utterance critique black star huge number of uh uh mike really
00:09:33
crazy not to read this heretic blacks and of course in i say this in the lever in in the bank
00:09:41
so here is an electron microscopy picture off of
00:09:45
patients that uh i took you recognise
00:09:47
uh here at the platform membrane off any better sideways the mic loose with the mitochondria
00:09:53
your mitochondria again and hear your recognise the the red dot sounds so here is this in is it
00:10:01
here you can see many degrees and if you use a higher magnification is then
00:10:06
you realise that this the brace on of the boy is this all musicals
00:10:12
and the size is in the run of micro physicals
00:10:18
here is another intern because the big picture
00:10:21
if you are familiar with extra microscopy you can recognise generator side here here
00:10:26
and you got to say is the nucleus and with the mitochondria
00:10:30
here is enigmatic straight cell containing a becoming a year or two percent and
00:10:35
here you can see us from a small cell between all the sadness
00:10:39
and if this is another sickle cell and if you use uh how many
00:10:43
vacation you can see that the cell is releasing an express illogical
00:10:47
that might go to another send and leave a message to a neighbouring sent from
00:10:55
so let's move to uh michael musicals in the last ah of patient and
00:11:00
i will show you that we could make a missing my markers
00:11:05
this id started a few years ago and um
00:11:09
and you poking d. to a measure
00:11:12
mm mm plus my my commas goes from a patients wheeze a series is
00:11:17
but we do can or how many clad and uh from
00:11:20
healthy controls and we measured during uh using frostbite dimitri
00:11:25
micro visitors from a disease patients and since controls and uh uh we
00:11:31
before i'm a two characterisation and we were able to a measure
00:11:37
mike ridiculous off really cool on the serial already because this marker is
00:11:42
i'm not specific user for look aside or on the solar cells
00:11:47
from really can side from info site andy richter side and we saw that
00:11:52
one simple operations where increased in a patients always yours is and also
00:11:58
um we observe that the where quite an amount of data site micro basically think
00:12:03
patients resources while uh almost no can could be detected a in healthy controls
00:12:11
so it was uh the first interesting result then we looked at a dire is
00:12:17
a child puke scroll to have the the the the relationship with the severity
00:12:22
and we saw an increase with the severity of zero these of my critical afrikaners
00:12:26
it already in an off my critical off but the site or e. g.
00:12:32
and even more we so that uh it look my critical awfully consider reading aware
00:12:37
also stated we is that patients should label independently off che puke score
00:12:43
so based on these uh results we
00:12:47
uh obtain funding to perform another
00:12:50
study to try to body that properly in a prospective study if
00:12:55
uh extras to law physicals and particularly about aside my
00:12:58
cover the 'cause where associated with patients survivor
00:13:02
so with we perform these uh yesterday uh we surpass cohort with station
00:13:08
i'm from both almost be ten from our centre an evaluation cohort
00:13:13
that has been perform with uh the um i'm gonna need a unit imbecile enough uh uh
00:13:19
but i think for this occasion a so it was a nice
00:13:22
a collaboration and you can see that uh the inclusion
00:13:27
the patient included where uh uh be different between two cohort emotional
00:13:32
so we're mainly hardcore related charities well enough to know where where uh
00:13:37
mainly it today d. c. related as zeros is have to say
00:13:40
that all the stations where stable patients norris and acted even newbie d.
00:13:45
no real interagency no uh um infection except ross table patients
00:13:52
i think it's more frequently move on a bit less in missouri nah a. g. c.
00:13:56
more indoors or less muscle nah and uh you take you wear out was um
00:14:01
a you mean most of division in about an hour and it was a bit once on off each in a bottle
00:14:11
so if first reserved a impetus like micro physical level among the different stages uh
00:14:17
off here is is an as in the initial uh paper we observe that
00:14:22
michael physical uh uh for me but the sight reading increase with the severity of
00:14:26
series is most emotional and was in a in also in muscle now
00:14:33
then uh uh we um that i mean a threshold use using the you don't fest
00:14:38
and uh we found that the level of a sixty five you need the leader
00:14:44
can a separate to outcome which are significant
00:14:49
uh mm because patients who is
00:14:52
a high about the site level high sorry um my critical of a of a better
00:14:57
site uh in the broad have a poorer outcome this is six months survivor
00:15:03
uh we've computing recently these uh both in that escort and in the validation core
00:15:09
therefore it appears that the resulting weekly between about aside my
00:15:13
critical levels an outcome of the patients and then
00:15:17
we try to fit patients according to mel score to see if this is more uh mm uh uh
00:15:24
there is more information in c. v. r. on not to patients and we so that
00:15:30
it got to save my critical levels uh elevated play uh an important role in patients
00:15:36
with is a an alto both fifteens or four in b. c. your patients so
00:15:41
please keep that in mind that uh the um micro physical about separating are as sated with
00:15:48
patients survivor in severe cases it will be you will see link at the un
00:15:56
okay so michael was occurs uh are present in the broad of
00:16:00
patient with your resists menus of population make an increase and
00:16:04
uh we find that about aside my critical levels are related to patient outcome
00:16:08
but why are these micro the consolidated in the lot of the patients
00:16:14
when we are talking about the concentration of something we have
00:16:17
to take into account user clearance or production abbas
00:16:22
what about the appearance of micro physicals so we assess that was a
00:16:26
unknown and for that we need a funny experiment with the physicians
00:16:31
uh he says sorry we took mike with the kids and
00:16:35
we a lot by this micro physically with no no
00:16:38
particles this is there there is more bits of iron you can see here
00:16:44
uh my critical decorated with iron and then we
00:16:48
injected z. is a microwave figures into mice
00:16:52
and perform enter i and as you know like innovations always a remote commented this when i run is
00:16:59
taken by in organs and you've got the decrease in the intensity of the signal so here
00:17:03
our mice you can recognise like innovation here this being here
00:17:08
uh is the um uh the back of the patients of the um the
00:17:11
mouse uh here is the lever and uh here is this the match
00:17:16
and you can see that after injection there is a decreasing
00:17:19
intensity in the screen and also in the lever
00:17:23
and i didn't show you the lines batteries also decrease in the lines are for the the uptake
00:17:27
your clearance off mike over the course of course in the lever in the speed and uh
00:17:31
in the rounds mainly in macrophages but also in on the sears sells in these organs
00:17:38
so possibly in patients with zeros these scenes theories them action
00:17:43
we can speculate that there is a decreased clearance
00:17:46
but what about production we say that my cover the goods are really is following
00:17:51
activation and up to this is there in patients with a series is
00:17:57
features suggesting that in fact there is yes the earning between
00:18:03
uh inflammation about disease and a micro physical levels
00:18:07
so in what we do is we uh analyst a micro physical levers
00:18:12
in peripheral vein from patients but also in it i think they
00:18:16
and we observe that delivers where higher in it i
00:18:19
think they designed in peripheral vein suggesting uh that
00:18:23
the um the source of these visitors was that you don't which was expected but we demonstrated that
00:18:29
and then saying to a collaboration with our but to reduce anyway but
00:18:32
id we uh the account she quantified uh is the logical
00:18:37
decisions in patients having a dog and uh and uh my critical
00:18:41
measurement and she quantified yesterday connectivity user mine or see here
00:18:49
she quantify fibre this is or uh my farmers it all
00:18:53
not so i don fibres is f. three uh f. already neck a overridden timers this
00:18:59
and she also was able to see some apple petite uh says uh uh in some years on not
00:19:06
and in all cases we so that patients we use a high activity
00:19:14
up to this in the lever but also more fibre these hide
00:19:17
higher levels off a a better site micro physical suggesting
00:19:21
that information and that both of these contributed to increase a micro physical diverse
00:19:27
and we also not eighteen both patient population uh features i associated with
00:19:33
um my critical levels and we found of course uh severity
00:19:37
score of zero this uh so the serum sodium
00:19:42
a. j. p. g. or a migration but features off information we are
00:19:46
uh associated with where they're incorporated with uh michael with the colours
00:19:53
the white uh i think that we can uh conclude that in
00:19:56
our patient with yours is michael basically levels are increased
00:20:00
possibly following a decrease clearance and likely following an increased
00:20:05
inflammation an apple do this within the lever
00:20:11
so i'm mike was a girls are a power by improvising buyer markers but do they play
00:20:17
a role in zero the spectral physiology and so um i guess that we is uh
00:20:25
a well known leaders in a prototype attention you i've here or if you are aware of the fact
00:20:29
of his energy off bottle it attention that includes first an increase in try putting christians with
00:20:35
uh you know fibres this but also on justin is is and a calculation activation possibly
00:20:42
and second even is a spanking evaluation and sickening relation leading
00:20:48
to a non increased both are to go through and port hypertension
00:20:53
and let's look at the possible roll off my critical in these events
00:20:59
um there are features out data suggesting that my critical can contribute
00:21:04
to lever fibres is just pay these example where also rose
00:21:08
uh incubate it back to sites wheeze fatty acids and it but
00:21:13
aside for when you think mission really is micro physical expressing
00:21:17
uh try and trace all my critical is is able to interact with the
00:21:23
air for four five sorry i just a face off macrophages and then
00:21:29
is able to induce the release of a inflammatory
00:21:32
meticulous and favour fibre didn't is is so
00:21:37
from that you can uh understand that my covers acres transfer miss h.
00:21:42
from about aside to neighbouring cells and the way of interaction in
00:21:46
this example this is a rare leyden receptor interaction with issue i
00:21:52
will show you in a few seconds also way off interactions
00:21:57
and all those things so like under certain texan and they are a a pretty can contact cells that are quite close
00:22:06
regarding your budgeting disease if you expose it but decides
00:22:10
to separated free a fatty i see it's
00:22:14
see the battle sites really is a micro as acres exposing than
00:22:19
in one and in that case michael was a curse
00:22:23
fuse fuses on the serial says and that's heaved won the civil said men in one
00:22:29
which induces and easy so in that case this is a different
00:22:33
way of interactions not the like under certain directions is a
00:22:36
fusion and zen micro physicals can give to the uh target set
00:22:41
of a manicured and then you're change uh the function
00:22:48
uh with the team of something to say we uh the most
00:22:51
trade you that uh in the lever a portal i'm sorry
00:22:57
by decks can activate portal maya for blast and this bottle
00:23:01
my from last can really is micro physical does that
00:23:05
contain v. g. f. and e. g. f. is able to act
00:23:09
on memory sense cells and activate through v. g. f. receptor
00:23:14
uh on vision is is so and again another demonstration that a
00:23:18
microwave makers can contribute to reliever on june is is
00:23:25
so to summarise about satan bottle my from last uh in use the vaunted in this
00:23:30
at that stage i would like to emphasise the fact that my covers acres
00:23:36
i'd like to do initially are the right from one cell and therefore zack
00:23:41
content in protein in leap it uh in even in nucleic acid
00:23:47
changes from the cell they are the right from in uh
00:23:50
in other words micro physical derive from any better side
00:23:54
do not have the same contends than my critical dram from right but said and
00:23:59
also my critical the right from another say that said but even mail
00:24:03
what is interesting leave the composition and also action off mike
00:24:07
americans depends on the stimulus initiating is yeah blading
00:24:11
in other words if you take on the seat and sales in your activities on the see themselves
00:24:15
with history three stimuli you will not have the same composition off micro physical part of
00:24:22
competition will become an but the part would be specific and this is really important to when you
00:24:26
want to assess because we have to uh take michael recruit from a a set then
00:24:32
setting and if you take my career to get the right from patient with like my about induction
00:24:37
it is the effect will not be the same as micro physical derive from patient resources
00:24:43
so let's go back to um my critical wheezing and selection and deliver
00:24:48
um my chorus acres are also procurement why are my critical pro quite a lot
00:24:55
you have to consider that quite relation activation of course implicate cognition factors
00:25:02
but cognition factors are present in the blood circulating all the time
00:25:06
and to facilitate the interaction between uh various regulation factors
00:25:11
you need a membrane facilitating the interaction this membrane can be
00:25:16
activated brackets but this number in can be also
00:25:19
a micro physicals because they expose that justifies i'm positive searing that a
00:25:26
a low is the uh uh greg asian of calculation factors
00:25:30
so my covers because our program but some micro visitors are highly providing pro quite good on this
00:25:35
is micro visitors expressing at the surveys tissue factor and i remind you that you should factor
00:25:42
is uh the main activator obligation fact cascade
00:25:46
disaffected basically is present in will places where you shouldn't be
00:25:51
and there's and the capture of the lever in the brain in the your tears on
00:25:57
nobody's ever is not she factor we seem uh the broad because of the way you activate regulation
00:26:04
oops
00:26:06
but uh in this is conditions like uh innovation we sure is this
00:26:12
that could be pushed factor expresses low level inman aside the group
00:26:17
uh of of you already demonstrated that some years ago we is an increased activity
00:26:23
of shekels in money aside inpatient with your these we series is security
00:26:28
and users who softly should factor in a patient resources within
00:26:33
the broad is microwave records and lots of that
00:26:37
i mean crazy in the concentration of micro physicals caring teacher factor in the blood
00:26:44
so could this just factor present in the broader affirmation resources contribute to the
00:26:50
now well known activation of go regulation s. eighteen with yours is
00:26:55
we test is that uh some years ago and to make a long
00:26:59
story short what we demonstrating using twenty make any matters is that
00:27:04
when there is a leader injury there is an accusation of tissue factor was in the lever
00:27:09
and this activation of recognition you within your reach to quite relation activation
00:27:15
directly and also through a tissue factor positive micro physicals
00:27:20
and we must repeat also that a mother's side even those are increased uh they're a
00:27:26
check darkly d. c. no they do not play a role in calculation activation
00:27:33
so it was for the intrepid peek a pot let's have a look at the role all possible
00:27:39
roar of micro physical on the uh strongly against any uh part of cotton have attention
00:27:47
we just did that some years ago by taking a the um route from
00:27:51
patient with zeros this and basically what we did is uh we even
00:27:56
incubate is just my critical from patient with arctic rings from right
00:28:01
and then we assessed muscular activity using a mm uh organ chalmers
00:28:08
and what we demonstrated is that micro physicals of station with zeros is in their
00:28:14
contraction uh to buy the constructive asians and there's facilitate as as a delegation
00:28:21
and the mechanism is that the my critical i able to shoes with on the set and said is
00:28:26
and when they fuse with on the serial says they give to when the serial set
00:28:30
is a substrate for the arsenic as it passed way and screw cops one
00:28:35
the new so really is of course process taking it was x. v. view uh
00:28:39
the experiment but also what we d. easy weekend we injected into nice
00:28:45
a micro basic years from patients uh we is a series this and we observe that
00:28:51
the mike was good i able to decrease appeal brought pressure of uh mice
00:29:00
the for a micro circulating macros information resources
00:29:05
uh i think uh nearly the list of the factors that uh
00:29:08
contribute to presentation uh uh and typically spanky presentation vision resources
00:29:15
so
00:29:17
i was very uh interesting my newspaper that was coordinated by uh whether
00:29:23
it be an uh i may or so uh the i think
00:29:27
and on my paper where uh america's and which is a pun 'cause spies in
00:29:32
meter has been a testy in patients we is a a competitive zeros this
00:29:39
and uh the mustard and measure a g. p. g. prior
00:29:43
to a beginning the treatment and after the treatment
00:29:47
an invasion with the lou or not very high a. g. p. g. below twelve in a in a matter of mccurry
00:29:54
there was no impact off uh this uh in a tour of cast as on
00:30:00
a d. v. d. but in patients we is your prototype
00:30:04
attention seeking uses a decrease in portal uh hypertension
00:30:09
and this is really reminiscent of what we observe the
00:30:14
in our studies where we included on the um
00:30:17
and they can call point of view only beijing wheeze a secure servers instead p. b. or c.
00:30:22
and also uh for reminiscent of the studies where we see
00:30:26
that to my critical of the better set the reading
00:30:29
um our stated we survival only in patients who is a
00:30:33
high email score so i have the feeling that
00:30:36
uh is is micro physical play a role uh in patients with advanced as yours is
00:30:42
so we would be very very interested by the phase three right try and that is finished and great
00:30:51
in conclusion a microwave because our our very small
00:30:55
basic also point one one micro meter
00:30:58
as a contain many things uh only pete's protein and nucleic acid
00:31:03
they just circulating levels are increase the eh in patients with
00:31:07
zeros is particularly by the side and michael physicals
00:31:10
uh they are early markers of organ the function and
00:31:16
in my view there are there are present um
00:31:20
continuing a lever injury and i think it could it could be just a marker
00:31:25
discriminative patient that has to be an ongoing either injury for some reason
00:31:31
from the patients that has no more even dorian will maybe re remain more stable
00:31:36
uh and micro suckers uh are implicated in the butt of his early part of you at the many stages
00:31:42
offer you but this is a progression fibres these i didn't this is coalition activation and portal hypertension
00:31:48
i would like to thank you for attention and saying the team and the guys are they do work
00:31:54
thank you very much
00:32:01
thank you
00:32:13
if
00:32:15
yeah and i think you just to do it right
00:32:22
i formation you say used it as as you get like this so
00:32:27
any bandage less as which we have right e. to that
00:32:33
one good reason mike that or you have another process
00:32:39
these triggering i. e. e. e. to formation like hammers e.
00:32:49
so uh uh the um the formation uh
00:32:53
include gates um uh for instance uh
00:32:58
it's not to um by chance i would say uh it implicates stimuli that in due season much
00:33:04
engine can some level was in the senate and changes in the um uh the sector skeleton
00:33:10
uh i do surveys of mike americans so mm uh it's
00:33:15
uh uh the i would say yes a regulated process
00:33:19
a very light up to this is regulated um it's not
00:33:25
just an injury uh for like a new courses
00:33:28
i would say so mm uh are there
00:33:33
cantor even that prevent the activation of uh the
00:33:37
release of micro basic or else uh if
00:33:41
it's hard to say it directly if they uh as our sings that
00:33:45
directly uh prevent vacation you take for instance the uh the guess personally bitter
00:33:50
uh and the principal desire for lead for even the reserve my critical but
00:33:55
i don't know if this is really a and then to my eye
00:33:57
on time my critical uh uh um a drag so um
00:34:03
uh how to to give the straightforward and sell it
00:34:10
mm
00:34:30
i think a lot of stuff
00:34:38
well i actually so
00:34:47
so
00:34:49
in e.
00:34:52
so probably not
00:34:55
oh
00:35:16
yes
00:35:18
so what do i know what i mean
00:35:25
send it in the butt on the leader
00:35:36
yeah i say well yeah you know what
00:35:44
the age
00:35:50
i sell for them
00:36:05
if it's
00:36:10
so uh the first um i'm not sure that they are in
00:36:15
the blood but byproduct this is nice um it's not
00:36:20
the the first and we've got to have discussion between is
00:36:23
that clearance of macros again or is that specific targeting
00:36:28
to belong to the speed except for and now we've got some friends who are uh working catastrophic have got
00:36:35
project indicated to that i considering that it's not a clearances penises uh targeting to this
00:36:41
being that changes in the differences et cetera so i would not consider that
00:36:45
it just there but uh by chance and what we see for instance on that
00:36:50
directly to of course it that it uh x. on uh the essence moreover
00:36:55
uh uh we have evidence showing that mike curve physicals can
00:36:59
be taken up even binders in italian out arteries
00:37:02
not on the the spin opinionated or a will of course this is
00:37:06
more amount but to the interaction is possible uh um and
00:37:11
i should you deep impact uh on this directly t. in zero this setting we got to the data
00:37:17
uh in a uh in nice with a mind of refuge in your bedroom where the impact
00:37:22
muscular activity strongly so in the in the that's good or bad
00:37:28
so mm but what the concerning the the fact of
00:37:33
being more specific um the technique yes please
00:37:41
yeah well
00:37:50
yeah so with the technique we use um
00:37:56
uh we are analysing only the larger micro physicals uh with the the user with that
00:38:02
now a new technique and raising the smallest one but which is interesting is that
00:38:07
uh it apparently does more one uh carry less information than anyone
00:38:14
it appears that the information is taken by the larger physicals and it said
00:38:18
uh microscopes and not by the exams i'm talking about about the psychology
00:38:24
um then we would be interested in uh was or about
00:38:29
the sidewalk quite comfortable with our um way of
00:38:31
detection we validated that in the summer studies so we are able population uh i think is a robust
00:38:38
then there are also say populations uh what about the the
00:38:42
person's without on the circuit circuit percent except raw
00:38:46
uh this is uh not strike or what they deserve it meets a a lot
00:38:51
of set that because they are less common these uh uh this sense
00:38:57
and um and also you have to find a marker
00:39:01
that is specific for the seller foraging which is not so strict a lot
00:39:06
uh if you want them marker in the broad specific up for two percent for instance
00:39:10
it would not be so easy so this is the the quite challenging said that that the
00:39:17
we we work on that uh and and then you
00:39:22
so i would not consider that they if they have just local action they can have also an action remote action
00:39:29
but definitive you have a re a look accent for sure and then
00:39:32
would be interested in micro physicals present locally within that you're
00:39:38
and i'm not so comfortable uh we is the result of study is taking it but aside
00:39:43
putting on the better side or whatever and then uh uh collecting my course records
00:39:49
because uh i'm not sure of the relevance of what you have a of my current occurrence
00:39:55
i would definitely prefer my crazy 'cause i've edited from the lever of mice are animals
00:40:00
uh the but it's kind of like with um isolation of set us
00:40:05
it's not a more even more difficult raising great isolationist stance
00:40:11
the common uh sure because and yeah it it all fixed up because and models so there's um
00:40:21
they pick it because and all the acts like directly on the buttons right it
00:40:26
doesn't act on any other little cell is not capture shots and the signal
00:40:33
for influencing other people cells came from that but it came from the
00:40:38
but aside from that but aside and you have a better site
00:40:42
and from perhaps that are being treated with the implication
00:40:46
and you have the the so called johnny take this to make them and you quit other cells
00:40:51
you can see that the information travels from one to the others and you got the
00:40:56
if you put it because central culture and you take after was like icicles
00:41:02
from these and you at my critical faction into cultures
00:41:06
other little cells we point so much else matters
00:41:11
and it looks like it is from this more
00:41:16
a a size macros that it that it so i'll buy it looks like
00:41:22
by him giving him prick aside what if i picked out of the
00:41:26
i quizzical stopped producing italy but i'm probably this is the way that transmit information
00:41:32
but cell types but these getting selected in the in the in um
00:41:37
you know ways i you just said but the evil also
00:41:43
we were curious to see whether we put the hat
00:41:49
the stunts i take a effects of direct you to modifying
00:41:53
systemic but not mix because from the paper impatient now
00:41:58
anything about the mechanism of action yeah but i was able eight
00:42:02
amoco related to changes in your resistance in in perhaps
00:42:06
it appears to be like the nine percent due to changes in houston's out without any
00:42:13
you may them exchange so that we uh i would object rating they did but
00:42:18
effects and well it's it's curious because we we got degrees of popped
00:42:24
out of out of the disciples and um happening usually sort of
00:42:30
of different kinds of microwaves because that's got a check so object but all these can be um
00:42:38
not related to the number but up the body of the actors calls okay but
00:42:50
and we don't you think i could crash uh because the the then fledgling group
00:42:55
published a paper uh showing that the americas and decreases fibres is as well
00:43:01
uh and the uh
00:43:06
and uh the the fake irksome was a acute or
00:43:10
chronic i mean it was unrelated fibre this or
00:43:18
oh okay i
00:43:26
oh okay i i
00:43:35
okay so you can show on that uh that ah that degree or the
00:43:40
amount of ah my christy calls coming from capital side increases in the
00:43:45
more ah advances stages of zeros is but in these stages yeah that lever
00:43:51
under seal it also more copyrights only losers they are fun extra
00:43:55
so do you think that they uh these increasing in
00:43:59
lot ah upside arrived ah ah micro bicycles
00:44:04
comes a remaining finance stuff in this area more or that they got the code ah
00:44:10
go through the end of that i mean any other way like this with an ethics
00:44:17
i don't know this is a very good question differently uh i mean on so to that but this is a good question
00:44:27
yeah i know i did as many not but you might have seen these uh interesting paper showing that it could be
00:44:34
interesting to discriminate the by uh an invasion with p. s. c. uh current events in a
00:44:40
laugh from uh uh the nine the uh standards is so but i i didn't
00:44:48
thank you very much for nice presentation when you treat patients with her
00:44:52
domain is there any change quantity quality of microscopes and node
00:44:59
uh no i'm not aware of in the study uh
00:45:03
uh i seen that uh uh mm mm no
00:45:08
i don't know i uh this is a good question
00:45:14
with this of studies that stern you know the question i might do short one
00:45:21
i'm pretty much interested in per nonstick markers liver disease and you have show that
00:45:25
these promising so that might be a role for looking at mike about bicycles
00:45:31
my question is does wasn't quite that that would use group with many different stages of people disease
00:45:36
and you show that the amount of michael bicycles is related to probe
00:45:40
news operation my question is did you try to look within
00:45:45
more compensated stage and the more decompose at this stage whether these changes whether the remains
00:45:50
or whether they're not only the amount to microscope but maybe they it did you g.
00:45:55
t. outside mike russell could be a differential way of study find those within
00:46:01
it should be but the stage the ongoing uh yeah so yeah this is a this is an excellent
00:46:08
question is exactly what we are working on at the moment we're uh going on both sides
00:46:14
the competitive patients take a patients allowed records to see a long table
00:46:20
patients euphoria that it's really patients uh that will be compensate
00:46:25
from the those wooden that eh and in most of your patients
00:46:29
and use a a spectrum if we are able to
00:46:32
uh identify those who will die or uh uh do a
00:46:36
and elegance function as compared to those uh with that
00:46:40
but yeah this is definitely the this is the next step yeah all the currents that i i i
00:46:50
yes uh then the basically we observe
00:46:54
then also uh the group um
00:46:58
of uh and should man helps german uh that the as expected our invasion with it
00:47:04
but it is your it it's yours is more people segment critical patients with
00:47:08
uh i call rate it's yours is more you to find related services for
00:47:12
instance it was expected that it has been a a shot up so
00:47:17
so more of that uh not so much we had
00:47:22
the feeling that uh it's euros these um
00:47:28
uh is more buttons i would say that was ah come abilities a meeting that if
00:47:34
you take for instance it's known that the tobacco distributing your art at attention
00:47:39
increase um my critical never that your population we do not accept that and we've got the feeling that series is
00:47:46
is a more uh put on that would say this page and then uh come up with these
Conference Program
Role of microparticles in portal hypertension
Prof. Dr. med. Pierre-Emmanuel Rautou, Hospital Beaujon, Departement of Hepatology, University of Paris Diderot, France
Dec. 6, 2018 · 6:04 p.m.
193 views
Clinical decompensation and acute-on-chronic liver failure: evolving concepts
Prof. Dr. med Paolo Angeli, University of Padova, Department of Internal Medicine and Hepatology DIMED, Padova, Italy
Dec. 6, 2018 · 6:54 p.m.
384 views
Recommended talks
Anticoagulation treatment for portal vein thrombosis in patients with cirrhosis: DOACs – are we still far from using them?
Prof. Dr. med. Andrea De Gottardi, Hepatology, Inselspital, University Hospital Bern
June 21, 2018 · 7:30 p.m.
628 views
Nutrition, metabolic health, cancer and NCDs
Elio Riboli, Imperial College London, England
Sept. 28, 2017 · 2:27 p.m.
570 views